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20/Aug/2019

Are you facing an issue during the implantation procedure (under your IVF treatment)? If yes, then LAH technique with IVF will be beneficial for you. LAH or Laser Assisted Hatching procedure is nowadays has become the most acceptable process in ART technique and is performed when a female has the issue with her implantation step in IVF.

Assisted Hatching is usually referred to as LAH, is a procedure where zona pellucida – the outer shell of the embryo is carefully opened with the help of micromanipulation.  It is an advanced method to hatch an embryo to get favorable outcome during implantation of the embryo into the uterus lining. Pregnancy is all dependent on successful implantation and once implantation has occurred, pregnancy soon takes place. The rate of pregnancy turns high with laser assisted hatching method with IVF. Several women before their embryo transfer choose LAH to ensure a positive result of implantation.

What is Zona Pellucida?

Zona pellucida plays a crucial role during the entire procedure of LAH method. Retrieved eggs (after completing the dose of fertility medication and injections) not yet fertilized with a sperm, has an outer shell- which is referred as Zona Pellucida, this shell only allow a single sperm that can perforate with it and enter into the cytoplasm of the egg for further cell division. When fertilization happens, that means, a sperm directly enters through zona pellucida reaching into the cytoplasm of the egg, the zona shell keeps the cells of the embryo together.

Once the sperm bind with the receptor of zona pellucida, one of the zona glycol-protein is liberated, which in turn, causes father sperm to let go more enzymes, now the zona pellucida dissolves in that area and the sperm finally gets all the way through the wall to the actual egg cell.

The zona pellucid has different functions achieving pregnancy that include in oocyte growth, protection throughout its development, fertilization, spermatozoa binding, not allowing polyspermy, blastocyst growth and prevention of premature implantation.

What if the embryo’s zona pellucida wall is hard? Obviously, in this case, there will be difficulty in achieving implantation. An embryo may not be able to hatch from its zona to attach to the womb of the recipient.  During LAH procedure, a small hole is created in the zona pellucid of the embryo and with the help of a laser beam and micromanipulation method, the outer side of the embryo is drawn out and this, in turn, smooths the path of embryo hatching and result in easy implantation.  Laser-assisted hatching technique is more rewarding for those women who are above 35.

The procedure of laser Assisted hatching during IVF with ICSI

IVF with ICSI treatment is all based on the insertion of single motile and active sperm into each egg (after the collection of the unfertilized egg from each ovary). By using the advance micromanipulation technique, fertility experts select the sperm individually and microinjected into each egg. Once the insertion is done, fertility specialists wait and check the sign of fertilization in each oocyte or egg. ICSI is best suitable for male infertility factor; where the male partner sperm is unfit to self penetrate with the egg.

LAH or Laser-assisted hatching is a technique, is always used during the process of IVF. This method is performed after the third or fourth day of the fertilization step. LAH is not at all a time-taking technique. During LAH technique with IVF in ICSI, a laser beam is employed on the outside shell of an embryo; many of the people think that during this technique, the embryo is in danger from this laser but it’s not like this. Here the embryo does not come directly in contact with the laser, so the embryo is out of any harm.

The beam is performed by using an infrared 1.48-μm diode laser. Zona pellucida gets softened by this laser beam and hence the opening of an embryo is enhanced after this laser technique.

Laser Assisted Hatching with IVF in ICSI treatment is not a delayed method; this hardly takes few milliseconds. Laser beam thins the wall of zona pellucida shell to around 10 microns. And once this shell is softened, embryo hatching easily happens.

If the embryo doesn’t hatch properly, implantation and pregnancy will not take place and hence it is mandatory for the implantation- hatching is a must.

Who can undergo IVF with LAH?

  • Women, who are above their fertility age (usually at the stage of 35-40) – can opt for IVF with LAH. As the woman’s age increases, the shell becomes harder and occurring trouble in implantation. So, basically, zona pellucida softness and hardness is based on the age of the woman. This is the main reason, why younger women do not opt LAH with their IVF treatment because their shell is gentle than elder women.
  • Women whose shell of an embryo is thick and unable to hatch naturally.
  • Women, who have high follicle stimulating hormone (FSH) level, can go for this method.
  • Couples, who have chosen frozen embryo transfer in their IVF (the process of thawing or freezing the embryos make zona pellucida hard)
  • Poor quality of the embryo and be short of energy and nutrients to outright the hatching process.

Success Rate and the Cost of LAH with IVF/ICSI 

If we talk about the success rate of LAH with IVF/ICSI, then must say it results in good live birth through ART technique. Somehow, the success depends upon the embryologist’s experience and fertility team too.

LAH is the delicate process that must be carried out in a sophisticated manner and thus it needs skillful hands for accomplishment. The success rate of LAH is basically depended on –

  • Age of the woman
  • Embryo quality
  • Clinic’s success rate
  • Embryologist experience and qualification

Go IVF Surrogacy is the golden platform, which unravels any case of infertility in a calm way and results best in success rate. Providing all the latest techniques with ultra-modern equipment during the medication, it is famed for its high success rate and delivery counts.  The cost of LAH in India ranges from INR 20,000 to INR 30,000 (excluding the charges of IVF/ICSI). LAH has been proven as the best method to increase the chance of attaining pregnancy.


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20/Aug/2019

IVF – an ART technique that includes fertilization outside of the human body. Couples, who have undergone or planning to undergo their IVF, would have been introduced to a term referred to as Assisted Hatching.

During IVF treatment, a methodology or technique called Assisted Hatching may or may not be performed to help the embryo for successful implantation in a woman’s womb. In the year 1990, this procedure is introduced to assist the implantation procedure during IVF treatment. This step is performed three days post-fertilization (once the embryo has had a few days to develop). Definitely, Yes! Assisted Hatching improves the success rate of IVF treatment.

Several clinics offer this medical procedure (assisted hatching) as a part of their IVF treatment. The cost of assisted hatching offered by clinic could be of additional cost or included in IVF. So, if a couple is going forward for their IVF treatment then here is some information, one must read before settling on IVF with assisted hatching. Go IVF Surrogacy is one of the foremost medical healthcare companies that serves best to those infertile couples who are seeking for the best fertility clinic and of course veterans specialist. It’s a leading brand in the field of all the fertility treatments.

 

Assisted Hatching- how the procedure works-

Assisted hatching, as said earlier, is a fertility technique and always used as a part of the IVF procedure. This facilitates embryo hatching and thus clear implantation process.

All the eggs are surrounded by a protein shell, termed as zona pellucida. Egg after getting fertilized with the sperm (during IVF fertilization process) is known as a fertilized egg. After the first sperm fuses with the egg’ wall and gets through the zona pellucida into the cytoplasm of the egg for fertilization, the shell becomes impenetrable and hard so that rest of the sperm cells did not get penetrates with the fertilized egg. Fertilized egg starts growing fast, dividing into many cells and soon it becomes an embryo.

A fertilized embryo is covered by a shell, referred to as zona pellucida. For a smooth implantation process, the egg must hatch and break out from the zona pellucida and gets attached to the lining of the uterus.

This is what happens during assisted hatching that means, an embryologist weakens the barrier around embryo at a single point. This is done with a micromanipulation technique. Once the zona is thinned, it tears to allow the embryo to hatch. Those embryos that do not hatch during IVF procedure are not allowed to transfer into the female’s uterus.

There are three methods of assisted hatching –

  1. LAH – Laser Assisted Hatching
  2. PZD – Partial Zona Dissection
  3. Acid Tyrode’s Solution

Laser-assisted hatching in India is one amongst the most accepted and modern technique in the Assisted Hatching process. Laser-assisted hatching in India, in short, referred to as LAH, involves the usage of a laser device and this device makes a precise opening in the zona pellucida. This procedure is one of the safest and uncomplicated procedures compared to all the rest of the Assisted Hatching method.

Suitable Candidate for Assisted Hatching –

Assisted hatching by Go IVF Surrogacy is performed under the veterans and super specialist of fertility team, providing a decent success rate to the patient. It has observed that embryos with a thinner zona pellucida had higher rates of implantation in IVF treatments. Assisted Hatching is suitable and beneficial for –

  • Women, who have crossed 35 and undergoing IVF treatment.
  • Couples who have had repeated failures and unsuccessful results at pregnancy after a few IVF cycles.
  • Couple’s embryos where a distinctively thick zona is observed by the embryologist.

The cost of assisted hatching in India –

Being one of the most commonly used procedures, the cost of laser-assisted hatching in India can range from INR 15,000 to INR 30,000 depending on the fertility clinic

Assisted hatching procedure with IVF is now a commonly used procedure in India and other countries. India, being one of the centre points of fertility treatments, thousands of international couple travels India just for their IVF treatment with assisted hatching. The main centre of attraction of India is its reasonable cost bringing forth all the advanced equipment and the latest methodology during the treatment.

The cost of Laser Assisted Hatching in India begins from INR 20,000 and can vary up to INR 40,000 depending on the fertility clinic. Although the procedure of assisted hatching was developed as a way to improve the chances of embryo implantation, nothing can be said about its efficacy or you can say it is not a guaranteed technique. No doubt, assisted hatching boosts the implantation rate during IVF but again the couple needs to wait for a pregnancy test to know their status. Many of the factors go into the success of Laser Assisted Hatching in India such as the method used by the fertility clinic, specialist team of fertility and experienced embryologist, and the health of the embryo.

 


GO IVF Surrogacy is the leading clinic which provides the solution to all types of infertility issues. In short, we can say this is one stop destination where all infertile couples can fulfill their dream of having an own baby.


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